Data-enriched characterization of mobile populations. Morphological information and facts from images can add towards the richness of flow cytometry details. One example is, the figure exhibits an in any other case common apoptosis assay working with Annexin V and PI, introducing mobile imaging to characterize cells in each population to expose morphologically unique characteristics. These insights couldn't have been gained from multiplex staining alone.
As samples are obtained over the Attune CytPix Flow Cytometer, the substantial-pace brightfield digicam captures and outlets photos of detected gatherings at a charge nearly six,000 visuals for every 2nd, depending on the flow rate and graphic sizing. For better overall flexibility, Attune Cytometric Software program enables you to regulate the impression seize frequency as needed and select a particular gate for impression seize, if necessary.
Mix acoustic focusing technological know-how with high-parameter analysis for flexible spectral unmixing and conventional workflows.
The award-profitable Invitrogen Attune CytPix Flow Cytometer combines acoustic concentrating that has a large-velocity brightfield camera for simultaneous large throughput flow cytometry and superior resolution brightfield imaging.
"An image isn't fairly worth 1000 dots, nevertheless it really really helps to see photos. It is great to see photos that correlate with staining. For instance, AnnexinV-beneficial cells which can be morphologically various than healthier cells, or CD14+ monocytes which might be bigger and a lot more textured than CD3+/CD4+/CD14- lymphocytes.
Listed here, a highly trained person has gated singlets confidently. Immediately after assessing the handbook singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate consists of greater than 4% aggregates.
Pair your Attune flow cytometer with the autosampler for elevated workflow performance, and increase robotic integration for max throughput.
Constant image high-quality even at higher throughput Acoustic focusing and also a large-pace digital camera combine to impression these T cells continuously at low or superior flow rates. Easily regulate aim and camera configurations to satisfy experimental requirements.
Early signs of this contamination experienced initially been dismissed as debris. Backgating to find out new populations. Backgating imaged cells to the Attune CytPix Flow Cytometer also permits you to use morphological functions to find interesting subpopulations that may not be apparent from flow cytometry details alone.
Perhaps most importantly, these gatherings incorporate cells of Plainly various phenotypes which could lead to incorrect conclusions about double favourable events (particularly in uncommon populations).
AI-pushed automatic image Assessment with the Attune Cytometric Computer software interprets event characteristics into distinctive morphology parameters that may be combined with conventional fluorescence and scatter parameters to reveal extremely reproducible population variations and exclusive subsets.
We Beforehand shown the power of imaging Automobile-T/Ramos mobile interactions. Allow’s examine just the inhabitants of greatest curiosity, the double beneficial gatherings, To find out more. We will now use prolonged morphological parameters (right here, circularity and skewness of intensity) to further more examine the options of such populations and refine gating on these gatherings, escalating information robustness.
Created-in dimensionality reduction instruments amplified resolution into populations. Fluorescently gated populations back-gated on to UMAP plot to substantiate envisioned inhabitants resolution in lysed full human blood. Consumer can see activities from Just about every cluster for Visible affirmation of mobile phenotypes.
No wash/no lyse leukocyte analysis with imaging. Cells were obtained from 24-hour-aged blood by dilution in one mM EDTA ( Specialist UX/UI allows users to train AI models for expanded applications. (A) The “Prepare Model” attribute provides annotation tools and true-time model instruction accuracy.
At the guts of each Attune Flow Cytometer lies our innovative acoustic focusing technological know-how, which utilizes sound waves to meticulously align cells inside the flow mobile. This modern strategy surpasses regular hydrodynamic approaches, causing more quickly processing speeds, Extraordinary details good quality, and increased sample throughput.
The Attune Xenith cytometer is really a substantial-parameter spectral analyzer, providing the two spectral unmixing and conventional flow abilities for comprehensive and functional mobile analysis.
The Attune CytPix Flow Cytometer enables end users to visualize samples for the duration of acquisition, click here validate the precision of gating methods, and help final results with trustworthy documentation for publication uses.
What gave the impression to be a homogeneous inhabitants is really extra numerous—an Perception that ought to be viewed as when interpreting effects.