Facts-enriched characterization of cell populations. Morphological details from visuals can increase into the richness of flow cytometry info. By way of example, the figure demonstrates an usually traditional apoptosis assay working with Annexin V and PI, including mobile imaging to characterize cells in Every single inhabitants to expose morphologically distinct functions. These insights could not are actually acquired from multiplex staining by itself.
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(B) RAW 264.7 macrophages will often be annotated inaccurately with regard to particle depend because of their complexity and noncircular borders. The amount of singlets readily available for Examination improves as soon as the consumer trains and employs a far more correct design.
The award-successful Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing by using a higher-velocity brightfield digicam for simultaneous higher throughput flow cytometry and significant resolution brightfield imaging.
"An image isn't really fairly worthy of 1000 dots, nevertheless it truly really helps to see visuals. It is excellent to view photos that correlate with staining. For example, AnnexinV-favourable cells which are morphologically distinctive than wholesome cells, or CD14+ monocytes which might be more substantial and a lot more textured than CD3+/CD4+/CD14- lymphocytes.
Right here, an experienced consumer has gated singlets confidently. After assessing the guide singlet gate, the CytPix image-derived parameter ParticleCount reveals this gate is made up of over four% aggregates.
Pair your Attune flow cytometer by having an autosampler for greater workflow effectiveness, and increase robotic integration for maximum throughput.
Dependable impression excellent even at significant throughput Acoustic focusing plus a high-velocity digicam combine to impression these T cells regularly at very low or significant flow prices. Simply regulate concentration and camera options to fulfill experimental prerequisites.
The Attune NxT cytometer could be the reputable workhorse get more info instrument your lab can depend on for regular and reliable efficiency. The Attune CytPix product contains a superior-velocity brightfield digital camera and AI-pushed impression Assessment program, with recent upgrades such as equipment Understanding resources and an intuitive user interface, enabling Improved facts precision, accuracy, and quicker insights into sample biology.
Perhaps most of all, these occasions have cells of Obviously various phenotypes which could lead on to incorrect conclusions with regards to double optimistic functions (specifically in uncommon populations).
AI-pushed automatic picture Examination Along with the Attune Cytometric Software translates function options into distinctive morphology parameters which might be combined with regular fluorescence and scatter parameters to reveal really reproducible inhabitants alterations and special subsets.
We previously shown the strength of imaging Auto-T/Ramos cell interactions. Permit’s take a look at just the population of best fascination, the double positive events, To find out more. We are able to now use extended morphological parameters (below, circularity and skewness of intensity) to even further analyze the features of such populations and refine gating on these occasions, growing information robustness.
Leverage the power of Superior technological know-how and innovation to accelerate your discoveries. Have confidence in during the Attune Flow Cytometer family to provide the trustworthiness, speed, and accuracy that established your work aside.
No clean/no lyse leukocyte Investigation with imaging. Cells were acquired from 24-hour-aged blood by dilution in one mM EDTA ( Skilled UX/UI permits people to teach AI styles for expanded purposes. (A) The “Train Product” aspect supplies annotation equipment and actual-time product coaching accuracy.
At the heart of every Attune Flow Cytometer lies our innovative acoustic focusing engineering, which makes use of seem waves to meticulously align cells throughout the flow cell. This modern technique surpasses traditional hydrodynamic procedures, leading to a lot quicker processing speeds, Excellent facts good quality, and enhanced sample throughput.
As the mobile line experienced Beforehand been analyzed on the Attune CytPix Flow Cytometer, the scientists went again to the images and were capable of document the microbial infection not less than 5 times earlier.
The Attune CytPix Flow Cytometer permits users to visualise samples in the course of acquisition, verify the accuracy of gating methods, and guidance final results with trusted documentation for publication applications.
What appeared to be a homogeneous population is actually more varied—an insight that ought to be considered when interpreting results.